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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
08/01/2021 |
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Actualizado : |
08/01/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
SPAGNOL, D.; GIOVANAZ, M. A.; CARRA, B.; ABREU, E. SOZO DE; FACHINELLO, J. C.; MALGARIM, M. B.; MELO-FARIAS, P.; PASA, M. DA SILVEIRA |
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Afiliación : |
DANIEL SPAGNOL, Universidade Federal de Pelotas, Facultade de Agronomia Eliseu Maciel, Pelotas, RS, Brasil.; MARCOS ANTONIO GIOVANAZ, Universidade Federal de Pelotas, Facultade de Agronomia Eliseu Maciel, Pelotas, RS, Brasil.; BRUNO CARRA, INIA - Instituto Nacional de Investigación Agropecuaria, Uruguay.; EVERTON SOZO DE ABREU, Universidade Federal de Pelotas, Facultade de Agronomia Eliseu Maciel, Pelotas, RS, Brasil.; JOSÉ CARLOS FACHINELLO, Universidade Federal de Pelotas, Facultade de Agronomia Eliseu Maciel, Pelotas, RS, Brasil.; MARCELO BARBOSA MALFARIM, Universidade Federal de Pelotas, Facultade de Agronomia Eliseu Maciel, Pelotas, RS, Brasil.; PAULO MELO-FARIAS, Universidade Federal de Pelotas, Facultade de Agronomia Eliseu Maciel, Pelotas, RS, Brasil.; MATEUS DA SILVEIRA PASA, Universidade Federal de Pelotas, Facultade de Agronomia Eliseu Maciel, Pelotas, RS, Brasil. |
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Título : |
Hand-held mechanical device improves thinning efficiency of peach trees. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Australian Journal of Crop Science, December, 2020, volume 14 (9), Pages 1519-1524.Open Access. Doi: 10.21475/ajcs.20.14.09.p2683 |
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ISSN : |
1835-2707 |
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DOI : |
10.21475/ajcs.20.14.09.p2683 |
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Idioma : |
Inglés |
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Notas : |
Corresponing author: mateus.pasa@gmail.com |
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Contenido : |
Abstract
The objective of this study was to evaluate the effect of mechanical-manual thinning at different developmental stages in the thinning efficiency and productive performance of ?Sensação? peach trees. The experiment was performed during the 2013 and 2014 growing seasons, in a commercial orchard located in Morro Redondo (RS), Brazil. Plant material consisted of 7-year-old peach trees grafted on Capdeboscq rootstock and trained as an open-vase system. The experiment was arranged as a randomized block design, with five three-trees replications. In order to reduce the effect of personal experience, the treatments and measurements were applied by the same person in a set of replications throughout the experiment. Treatments consisted of: control I [without thinning (WT)]; control II [hand thinning (HT) 40 days after full bloom (40 DAFB)]; MmT at full bloom (FB): 50% of open flowers; MmT at the end of bloom (EB): 80-100% of open flowers; MmT at petal fall (PF); and MmT at the green fruit (GF) stage (fruit with ~1 cm of diameter). The MmT was performed using a hand-held portable device. The parameters assessed were: percentage of thinning, fruit set, thinning time, work economy, production per tree, fruit mass, estimated yield and fruit size distribution. The use of the MmT at the stages tested reduces thinning time of ?Sensação? peach trees, resulting in labor saving, as well as increases the percentage of fruit in category (CAT) 1. The MmT when performed at GF results in a higher percentage of thinning. The treatment MmT at FB increases the average fruit mass. MenosAbstract
The objective of this study was to evaluate the effect of mechanical-manual thinning at different developmental stages in the thinning efficiency and productive performance of ?Sensação? peach trees. The experiment was performed during the 2013 and 2014 growing seasons, in a commercial orchard located in Morro Redondo (RS), Brazil. Plant material consisted of 7-year-old peach trees grafted on Capdeboscq rootstock and trained as an open-vase system. The experiment was arranged as a randomized block design, with five three-trees replications. In order to reduce the effect of personal experience, the treatments and measurements were applied by the same person in a set of replications throughout the experiment. Treatments consisted of: control I [without thinning (WT)]; control II [hand thinning (HT) 40 days after full bloom (40 DAFB)]; MmT at full bloom (FB): 50% of open flowers; MmT at the end of bloom (EB): 80-100% of open flowers; MmT at petal fall (PF); and MmT at the green fruit (GF) stage (fruit with ~1 cm of diameter). The MmT was performed using a hand-held portable device. The parameters assessed were: percentage of thinning, fruit set, thinning time, work economy, production per tree, fruit mass, estimated yield and fruit size distribution. The use of the MmT at the stages tested reduces thinning time of ?Sensação? peach trees, resulting in labor saving, as well as increases the percentage of fruit in category (CAT) 1. The MmT when performed at GF results in ... Presentar Todo |
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Palabras claves : |
DURAZNO; FOLLOW-UP THINNING; LABOR SAVING; MECHANICAL-MANUAL DEVICE; PRUNUS PERSICA (L.BATSCH); RALEO; THINNING TIME; YIELD. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14919/1/AJCS-2020-SPAGNOL.pdf
https://www.cropj.com/triches_14_9_2020_1506_1518.pdf
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Marc : |
LEADER 02646naa a2200337 a 4500
001 1061657
005 2021-01-08
008 2020 bl uuuu u00u1 u #d
022 $a1835-2707
024 7 $a10.21475/ajcs.20.14.09.p2683$2DOI
100 1 $aSPAGNOL, D.
245 $aHand-held mechanical device improves thinning efficiency of peach trees.$h[electronic resource]
260 $c2020
500 $aCorresponing author: mateus.pasa@gmail.com
520 $aAbstract The objective of this study was to evaluate the effect of mechanical-manual thinning at different developmental stages in the thinning efficiency and productive performance of ?Sensação? peach trees. The experiment was performed during the 2013 and 2014 growing seasons, in a commercial orchard located in Morro Redondo (RS), Brazil. Plant material consisted of 7-year-old peach trees grafted on Capdeboscq rootstock and trained as an open-vase system. The experiment was arranged as a randomized block design, with five three-trees replications. In order to reduce the effect of personal experience, the treatments and measurements were applied by the same person in a set of replications throughout the experiment. Treatments consisted of: control I [without thinning (WT)]; control II [hand thinning (HT) 40 days after full bloom (40 DAFB)]; MmT at full bloom (FB): 50% of open flowers; MmT at the end of bloom (EB): 80-100% of open flowers; MmT at petal fall (PF); and MmT at the green fruit (GF) stage (fruit with ~1 cm of diameter). The MmT was performed using a hand-held portable device. The parameters assessed were: percentage of thinning, fruit set, thinning time, work economy, production per tree, fruit mass, estimated yield and fruit size distribution. The use of the MmT at the stages tested reduces thinning time of ?Sensação? peach trees, resulting in labor saving, as well as increases the percentage of fruit in category (CAT) 1. The MmT when performed at GF results in a higher percentage of thinning. The treatment MmT at FB increases the average fruit mass.
653 $aDURAZNO
653 $aFOLLOW-UP THINNING
653 $aLABOR SAVING
653 $aMECHANICAL-MANUAL DEVICE
653 $aPRUNUS PERSICA (L.BATSCH)
653 $aRALEO
653 $aTHINNING TIME
653 $aYIELD
700 1 $aGIOVANAZ, M. A.
700 1 $aCARRA, B.
700 1 $aABREU, E. SOZO DE
700 1 $aFACHINELLO, J. C.
700 1 $aMALGARIM, M. B.
700 1 $aMELO-FARIAS, P.
700 1 $aPASA, M. DA SILVEIRA
773 $tAustralian Journal of Crop Science, December, 2020, volume 14 (9), Pages 1519-1524.Open Access. Doi: 10.21475/ajcs.20.14.09.p2683
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INIA Treinta y Tres (TT) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
17/05/2022 |
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Actualizado : |
02/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
PASSOS, J. R. S.; GUERREIRO, D. D.; OTÁVIO, K. S.; SANTOS-NETO, P. C. DOS; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ-OLIVERA, R.; CRISPO, M.; BEZERRA, M. J. B.; SILVA, R. F.; LIMA, L. F.; FIGUEIREDO, J. R.; BUSTAMANTE-FILHO, I. C.; MENCHACA, A.; MOURA, A. A. |
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Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; DENISE D. GUERREIRO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; KAMILA S. OTÁVIO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; P. C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; FEDERICO CUADRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; RICHARD NUÑEZ-OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay; MARIA JÚLIA B. BEZERRA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; RENATO F. SILVA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; LARITZA F. LIMA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; JOSÉ RICARDO FIGUEIREDO, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; IVAN C. BUSTAMANTE-FILHO, aboratório de Biotecnologia da Reprodução Animal, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; ARLINDO A. MOURA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil. |
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Título : |
Global proteomic analysis of preimplantational ovine embryos produced in vitro. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Reproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122 |
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ISSN : |
0936-6768 |
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DOI : |
10.1111/rda.14122 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com |
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Contenido : |
ABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated
with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro.
© 2022 Wiley-VCH GmbH. MenosABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational i... Presentar Todo |
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Palabras claves : |
Embryo development; In vitro fertilization; Mass spectrometry; Oocyte; Ovine; PLATAFORMA SALUD ANIMAL; Proteins. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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Marc : |
LEADER 04571naa a2200409 a 4500
001 1063149
005 2022-12-02
008 2022 bl uuuu u00u1 u #d
022 $a0936-6768
024 7 $a10.1111/rda.14122$2DOI
100 1 $aPASSOS, J. R. S.
245 $aGlobal proteomic analysis of preimplantational ovine embryos produced in vitro.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com
520 $aABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro. © 2022 Wiley-VCH GmbH.
653 $aEmbryo development
653 $aIn vitro fertilization
653 $aMass spectrometry
653 $aOocyte
653 $aOvine
653 $aPLATAFORMA SALUD ANIMAL
653 $aProteins
700 1 $aGUERREIRO, D. D.
700 1 $aOTÁVIO, K. S.
700 1 $aSANTOS-NETO, P. C. DOS
700 1 $aSOUZA-NEVES, M.
700 1 $aCUADRO, F.
700 1 $aNUÑEZ-OLIVERA, R.
700 1 $aCRISPO, M.
700 1 $aBEZERRA, M. J. B.
700 1 $aSILVA, R. F.
700 1 $aLIMA, L. F.
700 1 $aFIGUEIREDO, J. R.
700 1 $aBUSTAMANTE-FILHO, I. C.
700 1 $aMENCHACA, A.
700 1 $aMOURA, A. A.
773 $tReproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122
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